ImpECNano: Environmental impact of nanomaterials: exposure through their life cycle and ecotoxicity. [Impact Environnemental des Nanomatériaux]: exposition via leur cycle de vie et écotoxicité. (Dir. CEREGE, financement obtenu en 2009), projet soutenu et financé par le Grenelle de l'Environnement, Pôle Applicatif Toxicologie - Écotoxicologie. Responsable/scientific coordinator BBE: Pr. A. THIÉRY.
initiated by the consortium iCEINT [International Consortium for the Environmental Implications of the Nano Technology], a GDRI [Groupement de Recherche International] in relation with the Center CEINT (DUKE UNIVERSITY USA). The GDRI is supported by the French CNRS and the CEA (French Atomic Energy Commission), within the OMERES [Observatoire Méditerranéen de l'Evolution des Risques pour l'Environnement et la Santé] and INERIS Frameworks. The aim of the works is to correlate environmental fate and ecotoxicity to the life cycle of nanomaterials. Based on our existing network and results obtained, the overall aim of the ImpECNano proposal is to reinforce the human power and the technological aspect of the existing platforms, but also to address issues that are not taken into account in the funded projects. Then ImpECNano will focus on nanoparticles not or poorly studied in our funded project (i.e. silver nanoparticles, CeO2 TiO2) and further investigate specific biologic effects. First the early effects in terms of ecotoxicity and at the ecosystem level will be studied. This latter will be based on the use and development of specific biomarkers. At the cellular level, ImpECNano will focus specifically on the mechanisms of cellular transfer and mainly on the nano-particles-Proteins interactions. To the best of our knowledge these issues are poorly studied. Finally and even if some studies have reported that nanoparticles may exhibit genotoxicity, our project will aimed at determining the mechanisms at the origin of the genotoxicity.
ANR PNANO 2008-2011: ANR NANAN: NANo-plateforme multifonctionnelle dérivée d'Acides Nucléiques à visée biomédicale, projet obtenu et financé (Coordinateur: Pr P. BARTHÉLÉMY, INSERM U869), Responsable/scientific Coordinator BBE: Pr. A. THIÉRY.
In this project, the main goal of BBE team is to evaluate the vulnerability of continental aquatic invertebrates to nanomaterials. The NANAN proposal is focused at developing a new multifunctional oligonucleotide-based nanoplatform for both nucleic acids imaging and oligonucleotide delivery. NANAN is a competitive hybridization-based technology using Quantum Dots as the central nanoparticle imaging cadmium core. Here, we identify the flux of nanomaterials and nanoparticles, and follow their evolution in vitro and in situ in the living targets. The main topics are: (i) evaluation of dose- response relationships for QDs acute, (ii) study of the chronic toxicity Follow up of the QDs uptake and fate to know their target tissue and their spatial distribution (iii) study of their cytotoxic effects and possible inference with cellular mechanisms.
European COST: The laboratory participates to the european COoperation in the field of Scientific and Technical Research COST FA0901: Putting halophytes to work: from genes to ecosystems 2009-2013. The main objective of the Action is to collate existing knowledge of halophytes from gene function to ecosystems that will impact on conservation and management of saline environments and agricultural productions. Our goal is to create an interdisciplinary group of scientists to bridge gaps between disciplines by jointly exploring the biodiversity of halophytes, re-evaluating their uses as crops, including bioenergy, as sources of salt-resistance genes and for use in the restoration and rehabilitation of salinized or contaminated land. Researcher involved: Isabelle Laffont-Schwob.
ANR Marséco: Head/Coordinator: Isabelle LAFFONT-SCHWOB.
Contaminant fluxes and coastal ecosystems functioning in Provence: soil -water-biocoenose transfers" 2008-2012. This work aims to fill the gap of knowledge on the understanding of high biodiversity in a site characterized by tourism multitude, organic pollution of sea sprays and, inorganic pollution of the soil and intends to give a global understanding of the pollutant fluxes at the level of the different compartments (soil, water, plants) and the biocoenose relationships. This study assumes to give an integrative vision of the situation from Marseilles to Cassis to lead to plant conservation and multi-polluted soil restoration and, more generally, quantify the health hazard on this site.
NanoDEFI: Metallic Nanoparticles: aquatic Dispersion and Ecotoxicity in Freshwater Invertebrates, Coordonnateur: X. MOREAU (Financement obtenu en 2009). Collaborations UMR 6635-CEREGE et UPR 3118-CINaM.
This interdisciplinary project shows three complementary axes: (i) manufacture and collection of gold nanoparticles (1 - 50 nm) with controlled morphology; (ii) comprehension of the dispersion and degradation mechanisms in freshwaters; (iii) evaluation of their potential toxicity on key invertebrates of the freshwater food webs. Special focus will be on detritivorous Amphipod crustaceans living at the sediment-water interface. This crustaceans represent a good model for studying the bioconcentration and fate of these nanoparticles. In order to look at possible differences in interspecific sensibility, the work will be carried out on 2 species: one of the Gammarus genus, exclusively located in Europe, and one of the Hyalella genus, exclusively found on the American continent.
|